Molecular Characterization And Genetic Improvement

Molecular Screening Of Elite/Plus Tree Of SaracaAsoca (Roxb.) De Wilde From Natural Population Of Similipal Biosphere Reserves

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  • Comparative account of Morphological and Biochemical parameters pertaining to identification of elite/plus trees of Saracaasoca from natural populations of five locations in Odisha.
  • Comparative account of RAPD and ISSR analysis pertaining to evaluation of degree of Polymorphism among different natural populations.

Salient Achievements

In order to select elite/plus tree of Saracaasoca, 4 morphological and 3 biochemical parameters were considered for comparison among 34 individuals collected from Similipal Biosphere Reserve, 21 individuals from Puri-Bhubaneswar, 57 individuals from Tamana, 36 individuals from Kapilash and 38 individuals from Champagarh Protected Forest regions. There was a lot variations among the plants collected from different populations. Comparison between morphological and biochemical data revealed that sample no 25 and 26 from Similipal Biosphere.

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Reserve (Table 1), sample no. 11,17 from Puri-Bhubaneswar, sample no 4,5 from Tamana ; sample no 8 and 15 from Kapilash and sample no. 15,23,36 from Champagarh region found to be superior.

Table:1. Comparative account on morpho-biochemical parameters for selection of elite/plus tree from Similipal Biosphere Resverve

Molecular analysis by 9 ISSR marker was carried out with 27 samples from Tamana and 28 samples from Kapilash reserve forest. The result revealed that % Polymorphism in Tamana (70.37) was higher than Kapilash (62.35). In Tamana region, 81 fragments were generated by using 9 ISSR marker out of which 57 bands were polymorphic with an average of 6.33. In Kapliash reserve forest, 85 fragments were generated by using 9 ISSR marker out of which 53 bands were polymorphic with an average of 5.88.

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Comparison of RAPD and ISSR marker showed that % Polymorphism is 70.37 for Tamana samples and 62.35 for Kapilash when analysed by ISSR marker. On the other hand, RAPD marker analysis showed % polymorhism 85.7 for Tamana plants and 93.22 for Kapilash samples.

Microbial Characterization And Industrial Application

Development OfBioinoculatns For Some Tree Legumes Useful In Revegetation Of Mine Area

  • Isolation and characterization of phosphate solubilising microbes from mines
  • Screening and selection of phosphate solubilisers as nursery inoculants for tree legumes

Salient Achievements

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Odisha is rich in variety of mineral resources i. e. bauxite, chromite, coal, fire clay, iron, lime stone, manganese, etc. The microflora of three mines soils i.e. Sukinda, Joda and Talcher was studied and 257 bacteria and 69 fungi were isolated and characterized for their various extracellular properties through one of the research project sanctioned by the Ministry of environment and Forests, Govt. of India (23/13/2001-RE 27th April, 2004 “Analysis of microbial community in heavy metal contaminated soils around mine site of Odisha”-2004-2006). Among them some potent phosphate solubilisers, Fe ore solubilisers, enzyme producers and antifungal organisms were obtained.

Image result for Microbial Characterization And Industrial Application The application aspect of these microbial strains especially mineral solubilization in individual and /or combination under glass house and field conditions with respect to enhancement in plant productivity and transplantation stability of some legume trees (recommended for the plantation in mine area due to their stress tolerance properties towards heavy metals) , were the main objective of this project.

Studies on Rhizoibum was worked out with isolation and/or screening protocols. In second phase, the efficiency of selected potent strains endowed with phosphate solubilization and uptake properties, iron solubilization, and nitrogen fixers was evaluated under glasshouse condition with respect to their effects on plant productivity in individually, co inoculation and combined inoculation conditions.

Image result for Microbial Characterization And Industrial ApplicationImpact of six different phosphate-solubilizing fungi and five bacteria and four iron ore solubilizing fungi were assessed in nursery conditions on tree legumes ( Acacia auriculiformis, A.nilotica, A. leucocephala , Dalbergiasissoo and Adenentherapavonina) was evaluated so that the treated plant could adapt to stress environment of heavy metal contaminated soils effectively. Third phase included was the preparation of nursery package /seedlings and transplantation into field soil conditions to analyze the field performance of the developed inoculants for better growth performance of selected tree legumes.

Fungal L AsparaginaseFrom Mangroves Of Bhitarkanika

  • Culutre and nutritional optimization for large scale production of L-asparaginase
  • Extraction, purification and characterization of L-asparaginases from fungi

Salient Achievements

L- asparaginase obtained from microbial sources have found wide applications in medicine as effective antitumor agent. The antitumor effect is attributed to their ability to suppress asparagine, which is important for tumor cell growth. L –asparaginase from bacterial origin sometimes cause severe allergies . Search of latest sources especially from fungal origin could also be useful within the development of potential drug. the bulk of L asparaginase is of intracellular in location, therefore total yield of asparaginase depends upon the cell biomass.

Image result for Microbial Characterization And Industrial ApplicationHence, the culture conditions and nutritional factors of L- asparaginase production were optimized for Aspergillus sp. the target of this experiment is to work out the L-asparaginase activity of Aspergillus sp. under different culture and nutritional conditions. Finally, the L- asparaginase extracted from cell biomass was purified through gel filtration and iron exchanged chromatography and characterized for its purity, molecular wt., km, thermal tolerance, pH requirement. The fungal species preferred starch and sorbitol as carbon and L- asparagine and L- glutamine as nitrogen sources.

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Addition of ionic compounds or basal nitrogen sources didn’t help in enhancing enzyme activity. Gel filtration and natural process chromatography produced pure enzyme which was later confirmed through PAGE exhibited 92.0 kda single chain peptide. L – asparaginase from the Aspergillus sp. preferred L asparagine as substrate, 7 pH , thermally stable upto 50C and showed 0.806×10-3 M Km. This study has been done under batch culture conditions. The media composition given within the present study are often further utilized in continuous fermentation for giant scale production of L – asparaginase.

In Situ And Ex Situ Biodiversity Assessment And Conservation

Salient Achievements

A five year project (2005-10) funded by DBT (Govt. of India) enumerated, identified and analyzed forest vegetation, its diversity, distribution and composition across 444 sample plots spread over 222 hacters in the district of Angul, Boudh, Bolangir, Cuttack, Ganjam, Kalahandi, Kandhamal, Khurda, Nayagarh and Rayagada districts. The simple plots were laid in dry deciduous, moist deciduous and semi evergreen forest zones occurring in the Eastern Ghat region of India (between 19062′ N – 21074′ N Lat.). In total 78,356 trees was measured along with collection of data of 257 tree species. There has been no earlier record of floristic diversity and population studies at landscape level in Odisha until the present one.

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In total of 884 plant species (263 species of trees, 78 species of shrubs, 138 species of climbers/ twinners and 405 herb species) were recorded from the Eastern Ghat region. They belong to 532 genera under 129 plant families and constitute 32.6% of plant species recorded over the whole of Odisha, which is estimated as 2,700 species (Saxena&Brahmam, 1994).

Out of 256 tree species enumerated from the study area in Eastern Ghat of Odisha, the 10 major species are Shorearobusta (IVI=17.36), Lanneacoromandelica (IVI=6.76), Madhucaindica (IVI=6.72), Diospyrosmelanoxylon (IVI=6.68), Terminaliaalata (IVI=6.02), Anogeissuslatifolia (IVI=5.96), Buchananialanzan (IVI=5.86), Schleicheraoleosa (IVI=5.85), Brideliaretusa (IVI=5.76) and Syzygiumcumini (IVI=5.71) in term of frequency of occurrence, density and dominance (IVI= Importance Value Index).


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  • Quantitative assessment of the geographic distribution and population’s status of the plant resources of the Odisha portion of Eastern Ghats.
  • Identifying the threats on the plant resources and enlisting the threatened species.
  • Analyzing the spatial and temporal patterns of change in specific plant resources along the Eastern Ghats.

Top 20 Tree Species In The Area

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  • Shorearobusta
  • Lanneacoromandelica
  • Madhucaindica
  • Diospyrosmelanoxylon
  • Terminaliaalata
  • Anogeissuslatifolia
  • Buchananialanzan
  • Schleicheraoleosa
  • Brideliaretusa
  • Syzygiumcumini
  • Semecarpusanacardium
  • Cleistanthuscollinus
  • Cassia fistula
  • Protiumserratum
  • Pterocarpusmarsupium
  • Mallotusphilippensis

Genotype Size And Genetic Diversity Analysis

Distribution And Assessment Of Genetic Diversity Of Canes In Khurda District

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  • To undertake quantitative ecological study of the populations of 3 species of Calamus (C. guruba, C. viminalis and C. latifolius) and mapping their distribution in Khurda Forest Division.
  • To derive phylogenetic relationships among the species and to assess inter and intra-population genetic diversity in these species using molecular tools.

Salient Achievements

An attempt was made to make a quantitative assessment of the three dominant wild species of Calamus (C. guruba, C. viminalis and C. latifolius), mapping their distribution in Khurda Forest Division and documenting the population structure of these taxa based on extensive field survey. Eleven (11) nos. of sample plots, each of 100 X 100 m size were laid in Ankula, Baliganda, Badakhola, Jeripada, Raidiha, Selingpara, Khadijhari forests of Balugaon Forest Range of Khurda forest division characterized by semi-evergreen forests.

Image result for Genotype Size And Genetic Diversity Analysis In addition to earlier known 3 species of cane (Calamusguruba, C. latifolius and C. viminalis), occurrence of three more species such as Calamuslatispinus, C. caesia and C. rotang has been reported from forest of Khurda Forest Division. The locality of occurrence and distribution of the species and population structure of all the 6 species have been recorded.

Genetic diversity and molecular phylogeny was analyzed using ISSR markers, where exceedingly high polymorphism (96.34%) was observed. Molecular analysis established close relationships between C. viminalis and C. guruba and C. latifolius-C. rotang-C. caesius formed another cluster showing closer genetic affinity. Genetic diversity analysis and population genetic studies were conducted from 8 populations of Calamusguruba from 5 districts of Odisha using ISSR marker.

Image result for Genotype Size And Genetic Diversity AnalysisThe population genetic analysis revealed a very low genetic variability at species level as compared to other species having similar life-history traits. The highest polymorphism (20.31%) was found in Cuttack and lowest (6.25%) in Ghatikia population. The data revealed partitioning of 30.74% of the total genetic diversity within the population and 69.25% among populations. The study suggested that in view of higher genetic diversity, C. guruba population from Cuttack must be preserved and utilized as germplasm resources for ex situ conservation and as source of seed/ planting materials for reintroduction programmes.

Quantitative Assessment, Mapping Of Geographical Distribution And Study Of Population Structure Of Forest Species

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  • Enumerating the plant resources, their population levels and structure:- diversity, dominance, density of individual species.
  • Developing thematic maps of the density and distribution for all the species of economic importance and botanical interest (medicinal, NTFP, timber, oil-yielding, fodder, fibre and special plant groups).
  • Quantitative estimation of available resource levels of the plants and level of disturbance.
  • Identifying the threats on the plant resources and enlisting the threatened species for conservation action.

Salient Achievements

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Plant diversity and population inventory was made in 28 grids of 1000 m X 5m size (0.5 ha) representing five major forest types of Odisha i.e. I. Odisha Semi-evergreen forests; II. Tropical Moist Desiduous forests, III. Tropical Dry Deciduous Forest, IV. Central Indian Hill forest and V. Littoral and Tidal Swamp forest. No. of plants, GBH, height of all trees above 30 cm GBH were recorded. Diversity and density of herbs, shrubs, lianas and regeneration of tree species were assessed from 5 x 5 m size plots laid within transects. The stand density, basal area, diversity indices, regeneration potential, distribution of diameter classes will be calculated and compared to characterize a distinct forest type. The level of disturbance was assessed laying grids at different distances from village clusters. A total of 100 grids will be worked out under the project and finally the data on ecological and diversity parameters will be analyses.

Ethnobotanical Studies And Indigenous Knowledge

Survey Of Wild Tubers And Their Uses By Tribals Of Similipal Biosphere Reserve

To make an inventory of the wild tubers, roots, rhizomes etc. used by the tribal people inhabiting the Similipal Biosphere Reserve and their mode of use/ consumption.

Salient Achievements

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Due to its peculiar geographical location and edaphic and climatic variables, Similipal Biosphere Reserve is a hot-spot of plant biodiversity. It is inhabited by a large number of primitive tribal people belonging to ErengaKharias, Mankirdia, Kolha, Santal, Kharia, Bhatudi, Bhumija, Ho, Munda, Ganda and PauriBhunyans tribes. They depend on agriculture, wage earning, hunting, food gathering, and collection of forest produces such as fire wood, roots an tubers, leaves, fruits, medicinal plants, gum resin etc. for their livelihood. Traditionally, they rely on a number of wild plants to supplement their diet. They collect and gather a number of tubers, rhizomes, roots, leafy shoots, flowers and fruits and wild mushrooms as regular food materials and also at the time of food scarcity.

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A study was undertaken within the tribal villages of Similipal Biosphere Reserve during 2008-2010 and both qualitative and quantitative data were amassed from primary and secondary sources in several seasons of the year. the first data were collected through regular field surveys amid local people, group discussions, PRA and Key Informant Survey. Information on quantity of tubers and rhizomes collected, consumed and sold for every species and mode of consumption and use value were collected through interaction with local tribals.

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In the present work, ethno-botanical information on 36 wild plant species having edible tubers/ rhizomes and used by the tribals of SimilipalBiosphare Reserve have been recorded. Several species of Dioscorea and aroids are commonly used by all tribes. While Arisaematortuosum, Costus specious, Pureriatuberosa, Asparagus racemosus etc. were found to have medicinal value, Dioscorea spp. and Pueraria tuberose have good food value. The wild population of many tuberous species have been depleted due to destructive harvesting and overexploitation.

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