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Molecular Screening Of Elite/Plus Tree Of SaracaAsoca (Roxb.) De Wilde From Natural Population Of Similipal Biosphere Reserves

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  • Comparative account of Morphological and Biochemical parameters pertaining to identification of elite/plus trees of Saracaasoca from natural populations of five locations in Odisha.
  • Comparative account of RAPD and ISSR analysis pertaining to evaluation of degree of Polymorphism among different natural populations.

Salient Achievements

In order to select elite/plus tree of Saracaasoca, 4 morphological and 3 biochemical parameters were considered for comparison among 34 individuals collected from Similipal Biosphere Reserve, 21 individuals from Puri-Bhubaneswar, 57 individuals from Tamana, 36 individuals from Kapilash and 38 individuals from Champagarh Protected Forest regions. There was a lot variations among the plants collected from different populations. Comparison between morphological and biochemical data revealed that sample no 25 and 26 from Similipal Biosphere.

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Reserve (Table 1), sample no. 11,17 from Puri-Bhubaneswar, sample no 4,5 from Tamana ; sample no 8 and 15 from Kapilash and sample no. 15,23,36 from Champagarh region found to be superior.

Table:1. Comparative account on morpho-biochemical parameters for selection of elite/plus tree from Similipal Biosphere Resverve

Molecular analysis by 9 ISSR marker was carried out with 27 samples from Tamana and 28 samples from Kapilash reserve forest. The result revealed that % Polymorphism in Tamana (70.37) was higher than Kapilash (62.35). In Tamana region, 81 fragments were generated by using 9 ISSR marker out of which 57 bands were polymorphic with an average of 6.33. In Kapliash reserve forest, 85 fragments were generated by using 9 ISSR marker out of which 53 bands were polymorphic with an average of 5.88.

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Comparison of RAPD and ISSR marker showed that % Polymorphism is 70.37 for Tamana samples and 62.35 for Kapilash when analysed by ISSR marker. On the other hand, RAPD marker analysis showed % polymorhism 85.7 for Tamana plants and 93.22 for Kapilash samples.